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gfp cd63  (Addgene inc)


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    Addgene inc gfp cd63
    Gfp Cd63, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 93 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gfp+cd63/CD63-pEGFP+C2+(Plasmid+%2362964)/10__1091_slash_mbc__e25___04___0196-295-4-36
    Average 95 stars, based on 93 article reviews
    gfp cd63 - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    other:

    Article Title: STX1A localizes to the lysosome and controls its exocytosis
    Article Snippet: Lysosome exocytosis is one of the critical functions of lysosomes in maintaining cellular homeostasis and plasma membrane repair.. At the basal level, the SNAREs regulating the lysosome fusion with the cell surface have been poorly defined.. Here, we identified a Qa-SNARE STX1A, localized majorly to lysosomes and a cohort to the plasma membrane in HeLa cells.

    Article Title: STX1A localizes to the lysosome and controls its exocytosis
    Article Snippet: Other plasmids: mCherry-UtrCh (26740), GFP-CD63 (62964), LAMP1-GFP (34831), LAMP1-RFP (1817), pMD2.G (VSV-G lentiviral envelop vector, 12259), psPAX2 (lentiviral packaging vector, 12260), pmRFP-LC3 (21075) and ptLC3 (rat LC3 fused to mRFP and EGFP, 21074) were obtained from Addgene.

    Plasmid Preparation:

    Article Title: Lysosomal degradation of newly formed insulin granules contributes to β cell failure in diabetes
    Article Snippet: .. GFP-CD63 (CD63-pEGFP C2) was a gift from Paul Luzio (Addgene plasmid # 62964). ..



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    A. Cy5-miR-603 is released in CD63⁻ extracellular vesicles (EVs) larger than exosome. <t>CD63-GFP</t> expressing LN340 was transfected with Cy5-tagged miR-603 or miR-NT (Ctrl-miR) and treated with 6 Gy IR or mock treatment and monitored using live-cell fluorescence imaging. Left : representative images of EV release (actual video shown in Fig. S1A ). Blue box: CD63-GFP + EVs; white box: Cy5-miR-603 + EVs. Right : Quantification of Cy5-miR-603 + EVs across 100 fields. ***p < 0.001 between indicated groups (Student’s t-test). Scale bar is 10 μm. B. miR-603 is exported in Annexin V - vesicles. EVs are isolated from LN340 cells transfected with Cy5-tagged miR-603 or miR-NT (Ctrl-miR), treated with 6 Gy IR or mock treatment, and analyzed by flow cytometry. Left : Cy5/FITC-Annexin V contour plot. Red box: Cy5 + /Annexin V - EVs. Right : Quantification Cy5 + /Annexin V - EVs. ***p < 0.001 between indicated groups (Student’s t-test). C. Radiation induced export of endogenous miR-603. LN340 cells were treated 6 Gy IR or mock treatment, cultured for 12, and processed for RNAscope HD assay for miR-603. Left : representative images of nuclei counterstained with hematoxylin. Brown punctate signals indicate miR-603 detection. Right : the number of cytoplasmic and extracellular miR-603 puncta quantified from 5,000 cells treated with or without IR (left). The percentage of extracellular miR-603 puncta is shown (right). Data represent mean ± SD. ***p< 0.001.
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    A. Cy5-miR-603 is released in CD63⁻ extracellular vesicles (EVs) larger than exosome. <t>CD63-GFP</t> expressing LN340 was transfected with Cy5-tagged miR-603 or miR-NT (Ctrl-miR) and treated with 6 Gy IR or mock treatment and monitored using live-cell fluorescence imaging. Left : representative images of EV release (actual video shown in Fig. S1A ). Blue box: CD63-GFP + EVs; white box: Cy5-miR-603 + EVs. Right : Quantification of Cy5-miR-603 + EVs across 100 fields. ***p < 0.001 between indicated groups (Student’s t-test). Scale bar is 10 μm. B. miR-603 is exported in Annexin V - vesicles. EVs are isolated from LN340 cells transfected with Cy5-tagged miR-603 or miR-NT (Ctrl-miR), treated with 6 Gy IR or mock treatment, and analyzed by flow cytometry. Left : Cy5/FITC-Annexin V contour plot. Red box: Cy5 + /Annexin V - EVs. Right : Quantification Cy5 + /Annexin V - EVs. ***p < 0.001 between indicated groups (Student’s t-test). C. Radiation induced export of endogenous miR-603. LN340 cells were treated 6 Gy IR or mock treatment, cultured for 12, and processed for RNAscope HD assay for miR-603. Left : representative images of nuclei counterstained with hematoxylin. Brown punctate signals indicate miR-603 detection. Right : the number of cytoplasmic and extracellular miR-603 puncta quantified from 5,000 cells treated with or without IR (left). The percentage of extracellular miR-603 puncta is shown (right). Data represent mean ± SD. ***p< 0.001.
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    A. Cy5-miR-603 is released in CD63⁻ extracellular vesicles (EVs) larger than exosome. <t>CD63-GFP</t> expressing LN340 was transfected with Cy5-tagged miR-603 or miR-NT (Ctrl-miR) and treated with 6 Gy IR or mock treatment and monitored using live-cell fluorescence imaging. Left : representative images of EV release (actual video shown in Fig. S1A ). Blue box: CD63-GFP + EVs; white box: Cy5-miR-603 + EVs. Right : Quantification of Cy5-miR-603 + EVs across 100 fields. ***p < 0.001 between indicated groups (Student’s t-test). Scale bar is 10 μm. B. miR-603 is exported in Annexin V - vesicles. EVs are isolated from LN340 cells transfected with Cy5-tagged miR-603 or miR-NT (Ctrl-miR), treated with 6 Gy IR or mock treatment, and analyzed by flow cytometry. Left : Cy5/FITC-Annexin V contour plot. Red box: Cy5 + /Annexin V - EVs. Right : Quantification Cy5 + /Annexin V - EVs. ***p < 0.001 between indicated groups (Student’s t-test). C. Radiation induced export of endogenous miR-603. LN340 cells were treated 6 Gy IR or mock treatment, cultured for 12, and processed for RNAscope HD assay for miR-603. Left : representative images of nuclei counterstained with hematoxylin. Brown punctate signals indicate miR-603 detection. Right : the number of cytoplasmic and extracellular miR-603 puncta quantified from 5,000 cells treated with or without IR (left). The percentage of extracellular miR-603 puncta is shown (right). Data represent mean ± SD. ***p< 0.001.
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    gfp cd63 plasmid  (Addgene inc)
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    A. Cy5-miR-603 is released in CD63⁻ extracellular vesicles (EVs) larger than exosome. <t>CD63-GFP</t> expressing LN340 was transfected with Cy5-tagged miR-603 or miR-NT (Ctrl-miR) and treated with 6 Gy IR or mock treatment and monitored using live-cell fluorescence imaging. Left : representative images of EV release (actual video shown in Fig. S1A ). Blue box: CD63-GFP + EVs; white box: Cy5-miR-603 + EVs. Right : Quantification of Cy5-miR-603 + EVs across 100 fields. ***p < 0.001 between indicated groups (Student’s t-test). Scale bar is 10 μm. B. miR-603 is exported in Annexin V - vesicles. EVs are isolated from LN340 cells transfected with Cy5-tagged miR-603 or miR-NT (Ctrl-miR), treated with 6 Gy IR or mock treatment, and analyzed by flow cytometry. Left : Cy5/FITC-Annexin V contour plot. Red box: Cy5 + /Annexin V - EVs. Right : Quantification Cy5 + /Annexin V - EVs. ***p < 0.001 between indicated groups (Student’s t-test). C. Radiation induced export of endogenous miR-603. LN340 cells were treated 6 Gy IR or mock treatment, cultured for 12, and processed for RNAscope HD assay for miR-603. Left : representative images of nuclei counterstained with hematoxylin. Brown punctate signals indicate miR-603 detection. Right : the number of cytoplasmic and extracellular miR-603 puncta quantified from 5,000 cells treated with or without IR (left). The percentage of extracellular miR-603 puncta is shown (right). Data represent mean ± SD. ***p< 0.001.
    Gfp Cd63 Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gfp+cd63/CD63-pEGFP+C2+(Plasmid+%2362964)/pmc12333413__nl5c02804_si_001-38-10-12
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    cd63 gfp vector  (Addgene inc)
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    Addgene inc cd63 gfp vector
    A. Cy5-miR-603 is released in CD63⁻ extracellular vesicles (EVs) larger than exosome. <t>CD63-GFP</t> expressing LN340 was transfected with Cy5-tagged miR-603 or miR-NT (Ctrl-miR) and treated with 6 Gy IR or mock treatment and monitored using live-cell fluorescence imaging. Left : representative images of EV release (actual video shown in Fig. S1A ). Blue box: CD63-GFP + EVs; white box: Cy5-miR-603 + EVs. Right : Quantification of Cy5-miR-603 + EVs across 100 fields. ***p < 0.001 between indicated groups (Student’s t-test). Scale bar is 10 μm. B. miR-603 is exported in Annexin V - vesicles. EVs are isolated from LN340 cells transfected with Cy5-tagged miR-603 or miR-NT (Ctrl-miR), treated with 6 Gy IR or mock treatment, and analyzed by flow cytometry. Left : Cy5/FITC-Annexin V contour plot. Red box: Cy5 + /Annexin V - EVs. Right : Quantification Cy5 + /Annexin V - EVs. ***p < 0.001 between indicated groups (Student’s t-test). C. Radiation induced export of endogenous miR-603. LN340 cells were treated 6 Gy IR or mock treatment, cultured for 12, and processed for RNAscope HD assay for miR-603. Left : representative images of nuclei counterstained with hematoxylin. Brown punctate signals indicate miR-603 detection. Right : the number of cytoplasmic and extracellular miR-603 puncta quantified from 5,000 cells treated with or without IR (left). The percentage of extracellular miR-603 puncta is shown (right). Data represent mean ± SD. ***p< 0.001.
    Cd63 Gfp Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cd63 gfp gfp sevs  (Addgene inc)
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    Addgene inc cd63 gfp gfp sevs
    A. Cy5-miR-603 is released in CD63⁻ extracellular vesicles (EVs) larger than exosome. <t>CD63-GFP</t> expressing LN340 was transfected with Cy5-tagged miR-603 or miR-NT (Ctrl-miR) and treated with 6 Gy IR or mock treatment and monitored using live-cell fluorescence imaging. Left : representative images of EV release (actual video shown in Fig. S1A ). Blue box: CD63-GFP + EVs; white box: Cy5-miR-603 + EVs. Right : Quantification of Cy5-miR-603 + EVs across 100 fields. ***p < 0.001 between indicated groups (Student’s t-test). Scale bar is 10 μm. B. miR-603 is exported in Annexin V - vesicles. EVs are isolated from LN340 cells transfected with Cy5-tagged miR-603 or miR-NT (Ctrl-miR), treated with 6 Gy IR or mock treatment, and analyzed by flow cytometry. Left : Cy5/FITC-Annexin V contour plot. Red box: Cy5 + /Annexin V - EVs. Right : Quantification Cy5 + /Annexin V - EVs. ***p < 0.001 between indicated groups (Student’s t-test). C. Radiation induced export of endogenous miR-603. LN340 cells were treated 6 Gy IR or mock treatment, cultured for 12, and processed for RNAscope HD assay for miR-603. Left : representative images of nuclei counterstained with hematoxylin. Brown punctate signals indicate miR-603 detection. Right : the number of cytoplasmic and extracellular miR-603 puncta quantified from 5,000 cells treated with or without IR (left). The percentage of extracellular miR-603 puncta is shown (right). Data represent mean ± SD. ***p< 0.001.
    Cd63 Gfp Gfp Sevs, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gfp+cd63/CD63-pEGFP+C2+(Plasmid+%2362964)/pmc11934211-50-10-22
    Average 95 stars, based on 1 article reviews
    cd63 gfp gfp sevs - by Bioz Stars, 2026-09
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    Image Search Results


    A. Cy5-miR-603 is released in CD63⁻ extracellular vesicles (EVs) larger than exosome. CD63-GFP expressing LN340 was transfected with Cy5-tagged miR-603 or miR-NT (Ctrl-miR) and treated with 6 Gy IR or mock treatment and monitored using live-cell fluorescence imaging. Left : representative images of EV release (actual video shown in Fig. S1A ). Blue box: CD63-GFP + EVs; white box: Cy5-miR-603 + EVs. Right : Quantification of Cy5-miR-603 + EVs across 100 fields. ***p < 0.001 between indicated groups (Student’s t-test). Scale bar is 10 μm. B. miR-603 is exported in Annexin V - vesicles. EVs are isolated from LN340 cells transfected with Cy5-tagged miR-603 or miR-NT (Ctrl-miR), treated with 6 Gy IR or mock treatment, and analyzed by flow cytometry. Left : Cy5/FITC-Annexin V contour plot. Red box: Cy5 + /Annexin V - EVs. Right : Quantification Cy5 + /Annexin V - EVs. ***p < 0.001 between indicated groups (Student’s t-test). C. Radiation induced export of endogenous miR-603. LN340 cells were treated 6 Gy IR or mock treatment, cultured for 12, and processed for RNAscope HD assay for miR-603. Left : representative images of nuclei counterstained with hematoxylin. Brown punctate signals indicate miR-603 detection. Right : the number of cytoplasmic and extracellular miR-603 puncta quantified from 5,000 cells treated with or without IR (left). The percentage of extracellular miR-603 puncta is shown (right). Data represent mean ± SD. ***p< 0.001.

    Journal: bioRxiv

    Article Title: A SRC-Annexin A2 axis that couples membrane repair to microRNA export during radiation stress in glioblastoma

    doi: 10.64898/2026.04.21.719868

    Figure Lengend Snippet: A. Cy5-miR-603 is released in CD63⁻ extracellular vesicles (EVs) larger than exosome. CD63-GFP expressing LN340 was transfected with Cy5-tagged miR-603 or miR-NT (Ctrl-miR) and treated with 6 Gy IR or mock treatment and monitored using live-cell fluorescence imaging. Left : representative images of EV release (actual video shown in Fig. S1A ). Blue box: CD63-GFP + EVs; white box: Cy5-miR-603 + EVs. Right : Quantification of Cy5-miR-603 + EVs across 100 fields. ***p < 0.001 between indicated groups (Student’s t-test). Scale bar is 10 μm. B. miR-603 is exported in Annexin V - vesicles. EVs are isolated from LN340 cells transfected with Cy5-tagged miR-603 or miR-NT (Ctrl-miR), treated with 6 Gy IR or mock treatment, and analyzed by flow cytometry. Left : Cy5/FITC-Annexin V contour plot. Red box: Cy5 + /Annexin V - EVs. Right : Quantification Cy5 + /Annexin V - EVs. ***p < 0.001 between indicated groups (Student’s t-test). C. Radiation induced export of endogenous miR-603. LN340 cells were treated 6 Gy IR or mock treatment, cultured for 12, and processed for RNAscope HD assay for miR-603. Left : representative images of nuclei counterstained with hematoxylin. Brown punctate signals indicate miR-603 detection. Right : the number of cytoplasmic and extracellular miR-603 puncta quantified from 5,000 cells treated with or without IR (left). The percentage of extracellular miR-603 puncta is shown (right). Data represent mean ± SD. ***p< 0.001.

    Article Snippet: A CD63-GFP-expressing LN340 stable cell line was generated by transducing LN340 cells with CMV-CD63-GFP lentivirus (Creative Biogene).

    Techniques: Expressing, Transfection, Fluorescence, Imaging, Isolation, Flow Cytometry, Cell Culture, RNAscope, HD Assay