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gfp cd63  (Addgene inc)


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    Addgene inc gfp cd63
    Gfp Cd63, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 93 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gfp+cd63/10__1091_slash_mbc__e25___04___0196-295-4-36?v=Addgene+inc
    Average 95 stars, based on 93 article reviews
    gfp cd63 - by Bioz Stars, 2026-08
    95/100 stars

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    A. Cy5-miR-603 is released in CD63⁻ extracellular vesicles (EVs) larger than exosome. <t>CD63-GFP</t> expressing LN340 was transfected with Cy5-tagged miR-603 or miR-NT (Ctrl-miR) and treated with 6 Gy IR or mock treatment and monitored using live-cell fluorescence imaging. Left : representative images of EV release (actual video shown in Fig. S1A ). Blue box: CD63-GFP + EVs; white box: Cy5-miR-603 + EVs. Right : Quantification of Cy5-miR-603 + EVs across 100 fields. ***p < 0.001 between indicated groups (Student’s t-test). Scale bar is 10 μm. B. miR-603 is exported in Annexin V - vesicles. EVs are isolated from LN340 cells transfected with Cy5-tagged miR-603 or miR-NT (Ctrl-miR), treated with 6 Gy IR or mock treatment, and analyzed by flow cytometry. Left : Cy5/FITC-Annexin V contour plot. Red box: Cy5 + /Annexin V - EVs. Right : Quantification Cy5 + /Annexin V - EVs. ***p < 0.001 between indicated groups (Student’s t-test). C. Radiation induced export of endogenous miR-603. LN340 cells were treated 6 Gy IR or mock treatment, cultured for 12, and processed for RNAscope HD assay for miR-603. Left : representative images of nuclei counterstained with hematoxylin. Brown punctate signals indicate miR-603 detection. Right : the number of cytoplasmic and extracellular miR-603 puncta quantified from 5,000 cells treated with or without IR (left). The percentage of extracellular miR-603 puncta is shown (right). Data represent mean ± SD. ***p< 0.001.
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    A. Cy5-miR-603 is released in CD63⁻ extracellular vesicles (EVs) larger than exosome. <t>CD63-GFP</t> expressing LN340 was transfected with Cy5-tagged miR-603 or miR-NT (Ctrl-miR) and treated with 6 Gy IR or mock treatment and monitored using live-cell fluorescence imaging. Left : representative images of EV release (actual video shown in Fig. S1A ). Blue box: CD63-GFP + EVs; white box: Cy5-miR-603 + EVs. Right : Quantification of Cy5-miR-603 + EVs across 100 fields. ***p < 0.001 between indicated groups (Student’s t-test). Scale bar is 10 μm. B. miR-603 is exported in Annexin V - vesicles. EVs are isolated from LN340 cells transfected with Cy5-tagged miR-603 or miR-NT (Ctrl-miR), treated with 6 Gy IR or mock treatment, and analyzed by flow cytometry. Left : Cy5/FITC-Annexin V contour plot. Red box: Cy5 + /Annexin V - EVs. Right : Quantification Cy5 + /Annexin V - EVs. ***p < 0.001 between indicated groups (Student’s t-test). C. Radiation induced export of endogenous miR-603. LN340 cells were treated 6 Gy IR or mock treatment, cultured for 12, and processed for RNAscope HD assay for miR-603. Left : representative images of nuclei counterstained with hematoxylin. Brown punctate signals indicate miR-603 detection. Right : the number of cytoplasmic and extracellular miR-603 puncta quantified from 5,000 cells treated with or without IR (left). The percentage of extracellular miR-603 puncta is shown (right). Data represent mean ± SD. ***p< 0.001.
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    A. Cy5-miR-603 is released in CD63⁻ extracellular vesicles (EVs) larger than exosome. <t>CD63-GFP</t> expressing LN340 was transfected with Cy5-tagged miR-603 or miR-NT (Ctrl-miR) and treated with 6 Gy IR or mock treatment and monitored using live-cell fluorescence imaging. Left : representative images of EV release (actual video shown in Fig. S1A ). Blue box: CD63-GFP + EVs; white box: Cy5-miR-603 + EVs. Right : Quantification of Cy5-miR-603 + EVs across 100 fields. ***p < 0.001 between indicated groups (Student’s t-test). Scale bar is 10 μm. B. miR-603 is exported in Annexin V - vesicles. EVs are isolated from LN340 cells transfected with Cy5-tagged miR-603 or miR-NT (Ctrl-miR), treated with 6 Gy IR or mock treatment, and analyzed by flow cytometry. Left : Cy5/FITC-Annexin V contour plot. Red box: Cy5 + /Annexin V - EVs. Right : Quantification Cy5 + /Annexin V - EVs. ***p < 0.001 between indicated groups (Student’s t-test). C. Radiation induced export of endogenous miR-603. LN340 cells were treated 6 Gy IR or mock treatment, cultured for 12, and processed for RNAscope HD assay for miR-603. Left : representative images of nuclei counterstained with hematoxylin. Brown punctate signals indicate miR-603 detection. Right : the number of cytoplasmic and extracellular miR-603 puncta quantified from 5,000 cells treated with or without IR (left). The percentage of extracellular miR-603 puncta is shown (right). Data represent mean ± SD. ***p< 0.001.
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    A. Cy5-miR-603 is released in CD63⁻ extracellular vesicles (EVs) larger than exosome. <t>CD63-GFP</t> expressing LN340 was transfected with Cy5-tagged miR-603 or miR-NT (Ctrl-miR) and treated with 6 Gy IR or mock treatment and monitored using live-cell fluorescence imaging. Left : representative images of EV release (actual video shown in Fig. S1A ). Blue box: CD63-GFP + EVs; white box: Cy5-miR-603 + EVs. Right : Quantification of Cy5-miR-603 + EVs across 100 fields. ***p < 0.001 between indicated groups (Student’s t-test). Scale bar is 10 μm. B. miR-603 is exported in Annexin V - vesicles. EVs are isolated from LN340 cells transfected with Cy5-tagged miR-603 or miR-NT (Ctrl-miR), treated with 6 Gy IR or mock treatment, and analyzed by flow cytometry. Left : Cy5/FITC-Annexin V contour plot. Red box: Cy5 + /Annexin V - EVs. Right : Quantification Cy5 + /Annexin V - EVs. ***p < 0.001 between indicated groups (Student’s t-test). C. Radiation induced export of endogenous miR-603. LN340 cells were treated 6 Gy IR or mock treatment, cultured for 12, and processed for RNAscope HD assay for miR-603. Left : representative images of nuclei counterstained with hematoxylin. Brown punctate signals indicate miR-603 detection. Right : the number of cytoplasmic and extracellular miR-603 puncta quantified from 5,000 cells treated with or without IR (left). The percentage of extracellular miR-603 puncta is shown (right). Data represent mean ± SD. ***p< 0.001.
    Gfp Cd63 Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    A. Cy5-miR-603 is released in CD63⁻ extracellular vesicles (EVs) larger than exosome. <t>CD63-GFP</t> expressing LN340 was transfected with Cy5-tagged miR-603 or miR-NT (Ctrl-miR) and treated with 6 Gy IR or mock treatment and monitored using live-cell fluorescence imaging. Left : representative images of EV release (actual video shown in Fig. S1A ). Blue box: CD63-GFP + EVs; white box: Cy5-miR-603 + EVs. Right : Quantification of Cy5-miR-603 + EVs across 100 fields. ***p < 0.001 between indicated groups (Student’s t-test). Scale bar is 10 μm. B. miR-603 is exported in Annexin V - vesicles. EVs are isolated from LN340 cells transfected with Cy5-tagged miR-603 or miR-NT (Ctrl-miR), treated with 6 Gy IR or mock treatment, and analyzed by flow cytometry. Left : Cy5/FITC-Annexin V contour plot. Red box: Cy5 + /Annexin V - EVs. Right : Quantification Cy5 + /Annexin V - EVs. ***p < 0.001 between indicated groups (Student’s t-test). C. Radiation induced export of endogenous miR-603. LN340 cells were treated 6 Gy IR or mock treatment, cultured for 12, and processed for RNAscope HD assay for miR-603. Left : representative images of nuclei counterstained with hematoxylin. Brown punctate signals indicate miR-603 detection. Right : the number of cytoplasmic and extracellular miR-603 puncta quantified from 5,000 cells treated with or without IR (left). The percentage of extracellular miR-603 puncta is shown (right). Data represent mean ± SD. ***p< 0.001.
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    cd63 gfp gfp sevs  (Addgene inc)
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    A. Cy5-miR-603 is released in CD63⁻ extracellular vesicles (EVs) larger than exosome. <t>CD63-GFP</t> expressing LN340 was transfected with Cy5-tagged miR-603 or miR-NT (Ctrl-miR) and treated with 6 Gy IR or mock treatment and monitored using live-cell fluorescence imaging. Left : representative images of EV release (actual video shown in Fig. S1A ). Blue box: CD63-GFP + EVs; white box: Cy5-miR-603 + EVs. Right : Quantification of Cy5-miR-603 + EVs across 100 fields. ***p < 0.001 between indicated groups (Student’s t-test). Scale bar is 10 μm. B. miR-603 is exported in Annexin V - vesicles. EVs are isolated from LN340 cells transfected with Cy5-tagged miR-603 or miR-NT (Ctrl-miR), treated with 6 Gy IR or mock treatment, and analyzed by flow cytometry. Left : Cy5/FITC-Annexin V contour plot. Red box: Cy5 + /Annexin V - EVs. Right : Quantification Cy5 + /Annexin V - EVs. ***p < 0.001 between indicated groups (Student’s t-test). C. Radiation induced export of endogenous miR-603. LN340 cells were treated 6 Gy IR or mock treatment, cultured for 12, and processed for RNAscope HD assay for miR-603. Left : representative images of nuclei counterstained with hematoxylin. Brown punctate signals indicate miR-603 detection. Right : the number of cytoplasmic and extracellular miR-603 puncta quantified from 5,000 cells treated with or without IR (left). The percentage of extracellular miR-603 puncta is shown (right). Data represent mean ± SD. ***p< 0.001.
    Cd63 Gfp Gfp Sevs, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gfp+cd63/pmc11934211-50-10-22?v=Addgene+inc
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    Image Search Results


    A. Cy5-miR-603 is released in CD63⁻ extracellular vesicles (EVs) larger than exosome. CD63-GFP expressing LN340 was transfected with Cy5-tagged miR-603 or miR-NT (Ctrl-miR) and treated with 6 Gy IR or mock treatment and monitored using live-cell fluorescence imaging. Left : representative images of EV release (actual video shown in Fig. S1A ). Blue box: CD63-GFP + EVs; white box: Cy5-miR-603 + EVs. Right : Quantification of Cy5-miR-603 + EVs across 100 fields. ***p < 0.001 between indicated groups (Student’s t-test). Scale bar is 10 μm. B. miR-603 is exported in Annexin V - vesicles. EVs are isolated from LN340 cells transfected with Cy5-tagged miR-603 or miR-NT (Ctrl-miR), treated with 6 Gy IR or mock treatment, and analyzed by flow cytometry. Left : Cy5/FITC-Annexin V contour plot. Red box: Cy5 + /Annexin V - EVs. Right : Quantification Cy5 + /Annexin V - EVs. ***p < 0.001 between indicated groups (Student’s t-test). C. Radiation induced export of endogenous miR-603. LN340 cells were treated 6 Gy IR or mock treatment, cultured for 12, and processed for RNAscope HD assay for miR-603. Left : representative images of nuclei counterstained with hematoxylin. Brown punctate signals indicate miR-603 detection. Right : the number of cytoplasmic and extracellular miR-603 puncta quantified from 5,000 cells treated with or without IR (left). The percentage of extracellular miR-603 puncta is shown (right). Data represent mean ± SD. ***p< 0.001.

    Journal: bioRxiv

    Article Title: A SRC-Annexin A2 axis that couples membrane repair to microRNA export during radiation stress in glioblastoma

    doi: 10.64898/2026.04.21.719868

    Figure Lengend Snippet: A. Cy5-miR-603 is released in CD63⁻ extracellular vesicles (EVs) larger than exosome. CD63-GFP expressing LN340 was transfected with Cy5-tagged miR-603 or miR-NT (Ctrl-miR) and treated with 6 Gy IR or mock treatment and monitored using live-cell fluorescence imaging. Left : representative images of EV release (actual video shown in Fig. S1A ). Blue box: CD63-GFP + EVs; white box: Cy5-miR-603 + EVs. Right : Quantification of Cy5-miR-603 + EVs across 100 fields. ***p < 0.001 between indicated groups (Student’s t-test). Scale bar is 10 μm. B. miR-603 is exported in Annexin V - vesicles. EVs are isolated from LN340 cells transfected with Cy5-tagged miR-603 or miR-NT (Ctrl-miR), treated with 6 Gy IR or mock treatment, and analyzed by flow cytometry. Left : Cy5/FITC-Annexin V contour plot. Red box: Cy5 + /Annexin V - EVs. Right : Quantification Cy5 + /Annexin V - EVs. ***p < 0.001 between indicated groups (Student’s t-test). C. Radiation induced export of endogenous miR-603. LN340 cells were treated 6 Gy IR or mock treatment, cultured for 12, and processed for RNAscope HD assay for miR-603. Left : representative images of nuclei counterstained with hematoxylin. Brown punctate signals indicate miR-603 detection. Right : the number of cytoplasmic and extracellular miR-603 puncta quantified from 5,000 cells treated with or without IR (left). The percentage of extracellular miR-603 puncta is shown (right). Data represent mean ± SD. ***p< 0.001.

    Article Snippet: A CD63-GFP-expressing LN340 stable cell line was generated by transducing LN340 cells with CMV-CD63-GFP lentivirus (Creative Biogene).

    Techniques: Expressing, Transfection, Fluorescence, Imaging, Isolation, Flow Cytometry, Cell Culture, RNAscope, HD Assay